Waring, B.G., Averill, C., Bidartondo, M. et al. Microbiome manipulation and enhanced weathering influence tree growth in reforestation. Commun. Sustain. 1, 102 (2026).
The Glandwr Forest Carbon Study was launched in 2020 with the aim to maximise the carbon dioxide removal potential of forests, wherever reforestation is implemented.
Enhanced rock weathering significantly increased carbon removal, with young native woodlands storing up to 27% more aboveground carbon after four years.
Aboveground carbon (or aboveground carbon stock) refers to the woody biomass of the tree that is visible above the ground. Half of this woody biomass is made of organic carbon, an important ingredient in plant biomolecules like lignin and cellulose. The increase in aboveground carbon (or aboveground carbon stock) is calculated by summing individual tree biomasses in each research plot, and assumes that 50% of tree biomass is carbon.
When we look at all the native woodland research plots that received enhanced rock weathering, the trees, as a whole, stored an additional +27% of carbon in their woody biomass relative to control plots. The incremental carbon stored after four years was an additional 787.5 kg C ha-1.
When we look at the Sitka spruce research plots that received enhanced rock weathering, the intervention tended to increase individual tree growth, however there is no impact on aboveground carbon storage at the whole plot level after four years.
Further research is underway to assess the impact of enhanced rock weathering on organic carbon both above and below the ground.
During the first four years of the study, aboveground carbon was calculated using three tree measurements: diameter at base, tree height, and diameter at breast height (DBH) for trees over 1.3 meters. Data is quality checked, with any outliers remeasured.
These measurements, taken during the annual tree census known as the Big Tree Measure, are used to calculate aboveground carbon.
Both broadleaf and conifer species responded positively to enhanced rock weathering at the individual tree level, with the strongest responses by nitrogen-fixers (Alder) and trees with arbuscular mycorrhizal fungi species (Rowan and Cherry). At the whole-plot level, though, ERW had significant impacts on carbon storage for broadleaf plots only.
Silicate rock weathering is a natural geological process that already removes CO2 from the atmosphere. The ‘enhancement’ of rock weathering is done by crushing silicate rocks into smaller particles and spreading them over large areas, which accelerates their dissolution (i.e. weathering).
Enhanced rock weathering (ERW), in this case with crushed basalt, can boost tree growth via improved tree nutrition. There are two pathways for this: 1) directly releasing nutrients into the soil; and 2) increasing soil pH, which can make existing nutrients more available to the trees.
The crushed basalt acted as a vitamin shot (or fertilizer) for the trees by directly releasing calcium into the soil.
Findings showed that in the ERW research plots, plant tissue levels of calcium were elevated.
Researchers from Imperial College London independently estimated the rate of nutrient release by burying mesh bags containing crushed basalt in each of the ERW plots. One year later, the bags were analysed to quantify the loss of specific elements. The concentration of calcium in the bags declined by 44% in the bags due to the dissolution of various mineral types, including silicates (feldspars) and carbonates (calcite). The crushed basalt acted as a vitamin shot for the trees by releasing calcium into the soil.
The crushed basalt Increased soil pH making nutrients more available to the trees – improving access to nutrition.
At soil pH <6, the trees ability to access nutrients from the soil, such as Nitrogen, Phosphorus, Potassium, Calcium, and Magnesium, is reduced, with the strongest effect on Phosphorus.
Prior to the addition of the crushed basalt, soil pH values were the same across research plots. A year after the deployment of basalt, soil pH in rock-amended plots had increased, and was 5.65, compared with 5.30 in the control plots.
Across these plots, the overall amount of Nitrogen and Phosphorus in plant tissue also tended to increase. Both Nitrogen and Phosphorus were not present in the crushed basalt, the change in pH improved the trees’ ability to access these nutrients in the soil.
The growth response to the enhanced rock weathering intervention has continued across multiple applications of crushed basalt (2020 and 2023). This may indicate that the absolute amount of carbon sequestered in tree biomass due to ERW may increase as the forest ages.
Research at the Glandw Forest Carbon Study is continuing, with work underway to evaluate belowground and aboveground carbon at the five-year mark. Our aim is for this to be a long-term study and to monitor the impact.
In the plots where crushed basalt was added, the aboveground carbon stored in the woody biomass of the trees increased significantly by 2024: 17% for the enhanced rock weathering research areas as a whole, combining broadleaf and conifer forests, in comparison to the control plots.
The growth of individual tree stems was boosted by 9.6% on average with the crushed rock treatment. Nitrogen-fixing species (alder) and species with associated arbuscular mycorrhizal fungi (rowan and cherry) showed particularly strong benefits.
The effect on carbon stocks was bigger than the individual tree-level effect because: 1) the tree species which responded the most positively also made a bigger contribution to the plot-scale carbon stock (they are larger in size) and 2) the individual-level growth boost of the ERW treatment compounded (built on itself) year after year.
Silicate rock weathering is a natural geological process that already removes CO2 from the atmosphere. The ‘enhancement’ of rock weathering is done by crushing silicate rocks into smaller particles and spreading them over large areas, which accelerates their dissolution (i.e. weathering).
Rainwater contains dissolved atmospheric CO2. This rainwater reacts with silicate rock to form dissolved inorganic carbon (i.e. bicarbonate) which, if it is transported via rivers and groundwater to the ocean, can lock up that carbon for 1,000 years or more.
The weathering of the crushed basalt we applied was measured by looking at the changes in the chemistry of the raw material, retrieved from small mesh bags buried in the soil. On this basis, we estimate that the enhanced weathering process sequestered a maximum of 202-320 kg C ha-1 y-1. However, this estimate needs to be verified by quantifying the transport of the weathering products through the soil and into the nearby watershed – this work is ongoing.
Microbial enrichment showed the greatest impact on tree growth during the crucial first year after planting, with some species benefiting more than others, particularly spruce and oak.
Trees depend on a diverse community of root-associating soil fungi and bacteria to help them access nutrients. When trees are planted on land being repurposed for forestry, microbial enrichment can actively introduce beneficial microorganisms into the soil.
When we look at all the native woodland research plots that received enhanced rock weathering, the trees, as a whole, stored an additional +27% of carbon in their woody biomass relative to control plots. The incremental carbon stored after four years was an additional 787.5 kg C ha-1.
When we look at the Sitka spruce research plots that received enhanced rock weathering, the intervention tended to increase individual tree growth, however there is no impact on aboveground carbon storage at the whole plot level after four years.
Further research is underway to assess the impact of enhanced rock weathering on organic carbon both above and below the ground.
Variation in the responses by individual tree species may have been influenced by the closeness of the ‘match’ between the donor soil and the recipient trees. The conifer soil inoculum used was from a commercial forestry site with Sitka spruce, the broadleaf soil inoculum was from a native woodland with a predominance of oak.
Spruce and Oak trees that received microbial enrichment grew by +11% and +10% respectively over the first four years.
The results highlight the potential to harness soil microbial communities for afforestation and ecological restoration, although more work is required to optimise inoculum selection.
When a tree is moved from the nursery to its planting location is a very vulnerable time as roots establish in their new location.
Across all of the trees in the Glandwr Forest Carbon study, the trees that were largest after the first year, were the most likely to survive, or the least likely to die. The 25% of trees that were the largest were 86% less likely to die than the smallest 25% of trees.
Trees that received the microbial enrichment grew 45% larger than control trees in the first year, and this ‘fast start’ can play an important role in tree survival.
The size of this ‘boost’ declined through time, indicating that microbial enrichment is most important in the crucial first year of tree establishment.
A genetic fingerprinting method was used to show that some of the microbial species present in the initial inoculum had persisted in the soil, and were more abundant in the treated than control plots.
Research is underway with Royal Botanic Gardens, Kew looking at the role of mycorrhizal fungi at the nursery, in the soil inoculum and from existing trees in the landscape (eg hedgerows)
After four years, the broadleaf trees with microbial enrichment had stored 13% more aboveground carbon than those without this intervention, although the difference was not statistically significant due to high variability in tree growth across the landscape.
Aboveground carbon (or aboveground carbon stock) refers to the woody biomass of the tree that is visible above the ground. Half of this woody biomass is made of organic carbon, an important ingredient in plant biomolecules like lignin and cellulose. The increase in aboveground carbon (or aboveground carbon stock) is calculated by summing individual tree biomasses in each research plot, and assumes that 50% of tree biomass is carbon.
When we look at all the native woodland research plots that received microbial enrichment, the woodland as a whole tended to store +13% of carbon in their woody biomass relative to control plots. The incremental carbon stored after four years was an additional 383 kg C ha-1.
When we look at the Sitka spruce research plots that received microbial enrichment, the intervention increased tree growth, however there was limited impact (~20 kg C ha-1) on aboveground carbon storage at the whole plot level after the first four years.
Further research is underway to assess the impact of microbial enrichment on organic carbon both above and below the ground.
No benefits were found from combining interventions in the first four years.
The first four years of aboveground data in the Glandwr Forest Carbon Study found no benefit in combining nature based-interventions on tree growth, survival or carbon sequestered. Combining the two treatments created a distinct fungal community, and further research is required to assess the long-term impact.